Chapter 6 Making Vaccines
Wei Chen and a group of medical students from Nanding were busy among the wounded. While this battle shocked and frightened everyone, it also brought a new kind of trouble, which was the bacteria carried by the teeth of tigers and wolves.
This bacteria is somewhat similar to rabies, but even crazier than rabies. Those soldiers who were bitten quickly fell into a state of madness. Wei Chen could only use sedatives to calm all the wounded first.
Among the many wounded, the ones that were easier to deal with were those soldiers injured by their own bullets. Although they fell into the tigers and wolves, they were spared by the tigers and wolves because they were already injured, and they survived.
The poison department students have been arranged by Wei Chen to help all the soldiers on the front line. The vice-dean was invited to a meeting just now, leaving Wei Chen, the deputy captain, to sit in the tent.
Wei Chen drew blood from several bitten soldiers for observation. The rabies vaccine had been injected into the bodies of these wounded soldiers at the first time, but it had little effect. This new virus actually skipped the incubation period and
The prodromal stage directly enters the excitement stage.
You must know that the most difficult part of rabies is to survive the excitement period, but very few people can survive the excitement period. And the new virus in front of us, judging from the current symptoms, is more than twice as severe as rabies.
Wei Chen was about to turn around and enter the temporary laboratory to observe the blood, when a violent noise came from the large ward. Wei Chen looked back and saw that a wounded soldier who had just been injected with a sedative broke free from the rope that tied him
, and even unscrewed the guardrail of the alloy hospital bed and grabbed it in his hand. He banged the hospital bed frantically, and the surrounding medical students and nurses did not dare to approach.
A group of troops sent by the three major camps outside to protect Dr. Nanding rushed in after hearing the news. They raised their weapons and pointed them at their former comrade, tightly surrounding him.
Suddenly, the wounded soldier stopped knocking on the bed and turned his head. His eyes were all white, two tiger teeth protruded from his mouth, and all the blood vessels on his face were clearly visible. He stretched out his hand.
He scratched his neck, revealing his previously injured neck. The blood on the bite wound on his neck had stopped, and he wiped off the bandages and gauze originally attached to it.
After he roared at the crowd around him, he raised his head and stared closely at the white fluorescent lamp on the top of the large ward. After looking at it for less than three seconds, he raised his neck and spit out a large mouthful of deep black
blood, and then his body went limp and fell to the ground.
Wei Chen separated from the crowd and squatted down next to the wounded soldier. He stretched out two fingers of his right hand and pressed on the carotid artery of the wounded soldier. Then he shook his head. The wounded soldier actually died like that.
Wei Chen asked the students of the medical department to pay attention to the movements of the remaining wounded soldiers, and asked the soldiers responsible for guarding to stay in the large ward just in case. Then he returned to the laboratory again, and he had to research the corresponding measures as soon as possible.
of vaccines.
The rabies vaccine was developed by Pasteur in 1882 by weakening the toxicity of the virus through continuous passage of the virus and finally cultivating an adapted strain. This is the most primitive and most reliable method of making a vaccine, but now time is too tight and there are not so many.
Let Wei Chen cultivate the virus in his free time.
Wei Chen first poured a tube of blood into a large beaker of water nearby. He wanted to see if the virus could be directly separated from the blood through water. Taking advantage of this opportunity, Wei Chen took out another tube of blood and drew a little bit of it.
Observed under the microscope.
Through the microscope, Wei Chen saw black virus cells constantly destroying and infecting every blood cell in the blood. These black virus cells had two long flagella on their bodies. Every time they came close to a healthy cell, they would destroy it.
Two flagella penetrate the cell.
The blood cells that were penetrated by the flagella of the virus shrank violently for a while, as if they had lost their activity. Then they absorbed the surrounding nutrients and expanded again, and then slowly grew two small flagella from the inside.
, turned into a brand new virus cell.
These viruses themselves do not have the ability to reproduce, but instead create similar species through continuous infection.
Wei Chen looked at the microscope for a while and made a decision in his mind. Since it is a completely infectious type, there is no need to purify it. If left for a long time, all the cells will be virus cells. Wei Chen took out a 100ml syringe and pulled it up.
He took off his clothes, took off the weight on his waist, and then inserted the syringe into his right waist.
As Wei Chen exerted force, the syringe was slowly filled with blood. When Wei Chen pulled out the syringe, it was filled with bright blood.
Wei Chen put all his blood into a round-mouthed flask, took a little and put it into a glass dish, and then took out a little infected blood and dripped it in.
This is the method used by country Z to cultivate rabies vaccine. The rabies virus is inserted into the primary hamster kidney cells and then cultured to create a rabies vaccine. The original hamster Wei Chen didn’t know where to find it, so he had to extract his own kidney cells.
, I am invulnerable to all poisons now, so I can act as a guinea pig for a while, and there should be no problem.
The black blood and the red blood were slowly mixed together. The black blood was already the original solution of the virus. There was no such thing as insoluble blood types. Wei Chen stared at the glass dish closely. The red blood was once eaten away by the black blood.
As he looked, the two kinds of blood quickly mixed together and turned completely black. Wei Chen's eyes gradually became disappointed, can't it...
Wei Chen stood up and reached out to pick up the glass dish, ready to pour out the blood inside. However, the color of the blood in the glass dish slowly faded the moment Wei Chen reached out to grab it. Wei Chen slowly let go of his hand and poured it out again.
After observing it, I saw that the color of the originally black mixed liquid was constantly changing, from black to purple, then from purple to red, and finally stayed at bright red.
Wei Chen immediately put the glass dish under the microscope and saw that the originally snarling black viruses had all been replaced by a transparent thing with exactly the same appearance. Is this an antibody?
Wei Chen's face showed a joy, not only because he found a solution, but also because he had developed his own vaccine. Although he could not mass-produce it, although he relied on his invulnerable physique,
But it was a success after all.
The next thing was relatively simple. The dozens of blood that had just been drawn must have turned into virus stock solution. Wei Chen poured all the blood in those test tubes into the round-mouthed flask containing his own blood, and then
Chapter completed!