Chapter 63 The fifth generation that finally broke through!
Four days later.
Biomedical laboratory.
At this moment.
The entire laboratory was filled with an atmosphere of tension and excitement, and members of the research team wearing lab coats were constantly walking around quickly.
Not to mention a lively girl like Tang Yiqiu, a somewhat introverted and shy graduate student like Lian Ren Yongcun also had a look full of expectation flashing through his eyes from time to time.
In fact, the reason for the violent mood swings of these toolmakers is very simple:
After several days of overtime work, Xu Yun's previously conceived interference protein lure that can attract male cockroaches to interact with each other has been successfully born!
Now the research tasks of the entire research team have reached the final and most critical stage:
The attractant, the fourth generation imidacloprid, was synthesized through the combination formula deduced by Xu Yun, and tried to see if it could act on MC38 cells.
MC38 cells are one of the cells at the forefront of current biological research.
It is derived from the C57BL/6 mouse colon adenocarcinoma cell line, grows adherently and has fibroblast morphology.
When MC38 cells are implanted into C57BL/6 mice or mice with low immune function, tumors and metastases will form. Therefore, related technologies are mostly used to study the occurrence and metastasis of colorectal cancer, and have become one of the common ways to verify the efficacy of tumor drugs.
But this type of cell is not unique to mice; it is found in large numbers in cockroaches, dragonflies and praying mantises.
It belongs to a type of cell with relatively much research information in the field of gene editing.
And this is exactly why Xu Yun locked this anchor point:
First of all, it is related to the sodium ion channel of cockroaches and is an important electrical signal point for sodium ions.
Secondly, there are many relevant experimental results from various authorities in the biological world, so there is no need to spend a lot of experiments to derive research.
While Tang Yiqiu and others were busy outside, Xu Yun and Qiu Sheng were also standing in the operation room, making final preparations for the practical operation:
"Old Qiu, are you sure you can really do it?
Let me tell you, there is no room for error in this round of experiments. If you make a mistake, you have to start over from the beginning. Your hands cannot shake even a little bit - by the way, you didn't do it last night, right?"
"Don't worry, I didn't have time to watch Ria Yamanate, who fell ill last week...ah, what are you talking about?"
Qiu Sheng was concentrating on adjusting the equipment. He subconsciously replied half a sentence and then suddenly realized something was wrong and glared at him angrily:
"Come on, come on, Xu Shen, why don't you do it? I'll take a notebook nearby and take notes, okay?"
Xu Yun smiled when he saw this. He and Qiu Sheng were young, so they naturally knew whether the other party was really angry.
As the saying goes, there is a specialization in the field of science. Qiu Sheng is recognized as the first person among doctoral students at HKUST in directing group-assisted synthesis. Xu Yun's area of advantage is not in this direction.
A large part of Xu Yun's purpose of asking Qiu to come over for help lies in this current stage, so substitution is definitely impossible:
"Just kidding, hurry up and adjust the equipment and try to start it as soon as possible.
By the way, send me Ria Yamate’s novel later, I have a friend called a reader who wants to read it."
"..."
Material synthesis, this word may be familiar to many people, and it almost makes your ears go numb when you hear it.
But in fact, most people's understanding of this word only remains superficial and not in-depth.
For example, what is it like in a biological laboratory and what is the synthesis process?
If you really ask for detailed description of the steps in words, I'm afraid not even 1% of people will know.
Usually, material synthesis is generally divided into two concepts:
Synthesis of known substances and synthesis of unknown substances.
The operation method of the former is very simple. Just find intermediates through forward synthesis analysis and reverse synthesis analysis, which is high school knowledge.
As for the synthesis of unknown substances, it is more troublesome.
If the synthesis of unknown substances involves biological substances, it will inevitably involve polymerase chain reaction.
Through such a reaction, an originally extremely small amount of DNA molecules can be amplified tens to millions of times.
Then after design, it can be used to extract the required DNA information from trace amounts of biological samples, and can achieve the sensitivity of single cells or even single molecules.
At the same time, specific primers can also be designed to combine different DNA fragments to synthesize new DNA molecules.
This time, Xu Yun and others used a solid-phase chemical synthesis method, which uses solids such as resin as the substrate for the reaction, and the nucleic acid or protein molecules being synthesized are connected to it.
In this way, through continuous filtration and continuous replenishment of different raw materials for reaction, different monomer molecules can be connected to it in turn, and the required nucleic acid or protein can be synthesized according to the target sequence.
Twenty minutes later.
All preparations are completed and synthesis officially begins.
Xu Yun had previously deduced a total of five spatial structures formed by peptide chains, but Qiu Sheng and others only used one of them:
The C=O and N-H in the peptide plane are arranged in trans, and the peptide plane is rotated 27 degrees around Cα-N1.
After another five minutes, Xu Yun looked at Qiu Sheng:
"Lao Qiu, the biological pathway has been verified and the target cells have been locked. Let's proceed to the next step?"
Qiu Sheng asked without raising his head:
"Are the nucleotide monomers protected by DMT?"
"alright."
Qiu Sheng hummed and said nothing. He still believed in Xu Yun very much.
Then he turned his attention to a male cockroach that was immobilized on the console, and seriously started editing using CRISPR/Cas9 technology.
CRISPR/Cas9 technology was first proposed in 1987. In 2012, two female scientists, Jennifer Doudna and Emmanuelle Charpentier, discovered for the first time the endonuclease function mediated by Cas protein in the bacterial adaptive immune system. As a result, they also obtained
Received the 2015 Breakthrough Award in Life Sciences.
Since then, CRISPR/Cas9 has become the most mainstream gene editing system today.
Qiu Sheng locked the binding points calculated by Xu Yun. The target cells were MC38 cells from cockroaches. The purpose was to use hCD47-MC38 HuCELL to destroy the directional fragments that feedback gender recognition.
Once this fragment is bombarded, the cockroach's gender recognition will be affected.
Under the promotion of pheromones, the ketone good behaviors A+C mentioned earlier will be performed.
At this moment.
The internal microscopic world of that unlucky cockroach.
This is an extremely vast 'planet'. Under the yellow sky, it is filled with sinful scenes:
A spherical ball of pathogenic mycoplasma stepped on a piece of cholesterol under its feet, tore it into pieces with a hiss, and swallowed the broken limbs with a grin as the cholesterol howled.
Not far from it, another pathogen raised a newly synthesized Ig-like membrane protein over its head and violently dropped it off the cliff.
A little further away, a Coxsackie virus wielded its twin sickles and killed a sugar mutant...
Farther away is...
This is a world with no hope, no fairness, no hope, no...
future.
After the slaughter, the mycoplasma sat at the foot of the mountain, mechanically chewing the cholesterol in its mouth.
It has done this behavior countless times, and will continue until it dies.
However, just as it was looking for its next target, a sudden change occurred.
At some point, a black spot suddenly appeared on the originally yellow sky.
The black spot was very small at first, but in just one thousandth of a millisecond, it opened a huge hole.
Like a torn cloth, it expanded to the extreme distance of the field of vision.
Before Mycoplasma could react, something like an anchor suddenly appeared from the hole and rushed towards a fragment floating in the air.
Less than a moment.
The anchor hooked the fragment accurately and hooked it away from the world faster than when it came.
Mycoplasma, who had never seen such a situation before, looked at the situation in front of him in stunned silence.
Just as he was about to call his companions to inquire about the situation, a sound that sounded like a piercing sound suddenly came from his ears.
It subconsciously raised its head and looked at the sky.
At this moment, at the breach covering the sky, countless hot C1galt1c1 lures came through the air.
They came to this world with fire and justice.
"Gah?"
As soon as the pathogenic mycoplasma let out a strange cry of unknown meaning, it was smashed into pieces by the lure falling from the sky.
Actually, it's more than that.
At this moment.
All the sins in this world are being liquidated by divine punishment from the outside world.
In less than a moment, this sinful world was covered in flames and came to an end.
A few minutes later, the macro world.
As a series of changes occurred in the microscopic realm, the experimental cockroach that was returned to the ecological box suddenly spread its wings.
With a few flutters, he flew straight towards a male cockroach...
