Chapter 1016 The first vaccine, Grete, do you want to try it yourself?
One group conducts screening of weakly virulent strains.
One group is doing research on vaccine production methods.
One group is conducting research on vaccine preservation methods.
One group is conducting a study on vaccination methods...the experimental group uses [Epidemic Technique] to induce vaccination of the virus strain, and the control group uses injection to induce vaccination of the virus strain.
The 20 plague mages, as well as the mages who were originally in the research institute, were driven to a pulp by Gretel. Just preserving the vaccine exhausted the energy of that group of mages:
“Dissolve the vaccine in distilled water!”
"Dissolve the vaccine in sterile saline!"
"Use 0.5%, 1%, 2%,...5% glycerol solution to dissolve the vaccine!"
"Save at room temperature, save at four degrees above zero, save at zero degrees, save at minus 1 to 4 degrees, store at minus 20 degrees, and freeze-dry at minus 70 degrees!"
"Save it for one day, 5 days, 15 days, 30 days... half a year, a year!"
"Do all the combinations again!"
The key is that it is not done once it is done. After the time is up, the preserved vaccine needs to be taken out, thawed, diluted, and injected into the mice again. Then the mice are observed to see whether they are sick and whether they have obtained the vaccine.
Immunity…
"Are you going too far in training them like this?"
As Grete flipped through each set of reports submitted page by page, Archmage Heins couldn't help but complain:
"Even if you don't know exactly how to do it, at least you have a general idea! Are you going to let them try combinations one by one until they die?"
"I just don't know how to do it!"
Gretel spread out his hands confidently:
"I know how to do it. I started directly. What do you want them to do? I just don't know, so I ask them to try the plans one by one! Don't worry, when they make other vaccines next time, they will have these plans at least. It can be done.
Take it out and try again..."
"..."
"..."
No matter how Grete squeezed his labor force, the research and development of a live attenuated yellow fever virus vaccine finally yielded results bit by bit. The attenuated strain was stabilized and successfully passed the test of transmission for five consecutive generations. The virus genetics was stable and there was no sudden return of virulence.
powerful;
Research on vaccine production methods, using 7-day-old chicken embryos to inoculate the virus strain into the yolk sac, cultivating it at 37.5c~38c after inoculation, and harvesting it 70~80 hours, using a sufficient and stable supply of eggs at a low price to defeat mice
group and rabbit group;
(What? Cell group? Sorry, the cell group still failed to nourish the cells and missed the chance to participate in the competition)
The vaccine preservation group determined the optimal plan for harvesting chicken embryos and storing them at -40°C, absorbing the liquid to confirm sterility, grinding, centrifuging, and freeze-drying and sealing;
As for the vaccination group…
"Currently, it seems that the experimental group has fast vaccination speed and high efficiency. The disadvantage is that the vaccination effect is unstable." Grete flipped through the experimental records in his hand - the experimental records of inoculating the mice, and discussed with Archmage Heins and others.
:
"It may be that [Plague Spell] requires the control of a magician. Different magicians have different spell-casting intensities; even if it is the same magician, the intensity of each spell cast will be different due to the influence of mood and control level."
This difference in strength, so far, has not affected the protective efficacy of the vaccine - obviously, there is a lower limit to the strength of [Epidemic Technique] that can be stimulated - but it has affected its safety:
"The mice vaccinated using [Epidemic Technique] had an average of 1 adverse reaction in every 100 cases - including but not limited to local redness and swelling at the injection site, pain, poor feeding, fatigue, moderate fever, nausea, vomiting, diarrhea,
Twitching, etc.”
"Please, this is a disease spell!" Archmage Heins sneered:
"What other result do you want? It's just a little bit of discomfort, which is very mild compared to death from the illness."
"But the control group - that is, the group injected with syringes, is much safer." Grete turned another page of the information in his hand:
"So far, the adverse reaction rate is less than one in a thousand. In terms of vaccine safety alone, injection with a syringe is a better method."
"But the syringe is too troublesome." Linn said softly to Grete's right. He followed Grete from beginning to end on this project, sucking, injecting, sucking, injecting, and dealing with the syringe until he felt like vomiting:
"Disinfecting so many needles every day is a very troublesome thing... Even in the research institute, we have to inject millions of native people down the mountain. I don't think everyone will disinfect them well..."
"This is the troublesome part." Grete nodded lightly. In this world, no one knows better than him what will happen if syringes and needles are not sterilized properly——
In the world before time travel, even with the widespread use of barefoot doctors, even with the productivity of the 1970s and 1980s, and vaccination in mountain villages, there were still some places that were not strictly disinfected...
The result is a large-scale cross-infection. If one person in a village has hepatitis B or something, the whole village will often be infected...
The level of productivity and personnel training at that time was still so high. In this world full of natives, Grete couldn't even imagine it.
"Using a syringe will cause widespread infection...the disease in one person will spread to everyone..."
"Then what do you want?" Archmage Heins frowned. Gretel turned her face and stared into his eyes expectantly, her face full of matter-of-factness:
"I would like to ask you to try and develop some kind of inoculation device! Carve the spell model, put the standard strength magic crystal energy into it, and put the poison seeds into it -"
He gestured and pressed with his hand:
"Ka! The output is stable, the range is stable, and the adverse reaction rate is controlled at a very low range... Isn't this great?"
"..."
That's good, but why should this change-related thing be blamed on me?
Archmage Heins wanted to complain very much. However, under Gretel's expectant eyes, he still mumbled in response:
"Okay, I'll do this job. It's a necromancy spell after all. It won't bother me to dig a little deeper... Should we start now?"
"We have to wait a little longer." Gretel smiled:
"The vaccine still needs to be tested on monkeys. There is no problem with monkeys. The safety will be tested on humans. After the safety is confirmed, the effectiveness will be determined, and then large-scale vaccination will be carried out. At this last stage, it is the testing of epidemic techniques that will contribute.
opportunity……"
"Wait!" Archmage Heins looked at Gretel warily:
"Try it on people? You wouldn't be the first to try it on yourself, right? - I'm warning you, if you really dare to do this, I will take you back to see your teacher..."
If it was in his previous life, Gretel, following in the footsteps of his predecessors, might have actually given himself the first vaccine. But now, he just spreads his hands:
"My physique is at the level of a knight after all... if you hit me, the reaction will be very small, and the safety test will not be accurate!"
According to what you said, if you weren't physically strong enough, would you really want to test it on yourself? Archmage Heins couldn't help but roll his eyes:
"Besides, did you forget any items?"
"What?"
"The vaccine must be given to that snake god! If it uses disease to amplify your vaccine, one dose can be given to 100 people. Wouldn't there be less vaccines for you to sell?"
"This is also up to you!"
Grete spread his hands.
How to communicate with the snake god, how to train the indigenous god, how to get the maximum benefit from it...
For such a difficult job, if the 18th-level boss doesn't come forward, do you expect me to be a 10th-level novice?
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