Chapter 1280: Research reopens, blood-replenishing magic is too difficult!
No matter how much the elves screamed and howled, they could only hold on to the information given by Gretel and gnaw to death with difficulty and pain.
Half the time is spent chewing information, and the other half, I have to endure the torture in the laboratory:
"Aseptic operation! Aseptic operation! Don't pick up things that fall on the floor!"
Bang! The transparent hemostatic forceps flew out and accurately hit the radial styloid process of the offender, causing a whining sound.
"Who told you to put the lid down!"
Snapped!
The transparent hemostatic forceps were everywhere, and the elf who made the mistake was at least a seventh-level or eighth-level warrior, so he didn't escape.
"Don't touch the folded surface of the other glove with your gloved hand! Take off the gloves, go out and wash your hands, and put on the gloves again!"
Snapped!
The elves sighed, and so did Gretel. He really didn't want to train these elves if he didn't have to. God knows how difficult it is to have the sterile operating procedures ingrained into your bones and make every move and move without making any mistakes——
It is common for medical students who have just entered internship, even if they have been trained for several years, to be scolded or kicked out of the operating room;
It is also common for senior and mature doctors without special training to make mistakes when putting on and taking off protective clothing, face shields and masks, leading to self-infection;
As for ordinary people, the so-called wearing of masks, sterilization, and sealing off areas can basically only reduce the probability of being infected. To say that it is 100% guaranteed not to be infected, basically, cough...
All we can say is that if you get hit, it’s your fate, and if you don’t, it’s your destiny.
Of course, taking good protection in the face of the epidemic can reduce the chance of getting sick. Even if you accidentally get infected, inhaling the smallest amount of virus will take a longer time to replicate and cause illness, and it will also give the body enough time to react and synthesize antibodies.
The more antibodies there are, the less damage the human body will suffer, the symptoms will be milder, and the person will feel better...
It's just like Grete now. The more assistants he trains, the more assistants will help him do these low-end tasks, such as collecting umbilical cord blood from rabbits. As for the aseptic operation error, the extracted umbilical cord blood cannot be used?
Anyway, there are dozens of rabbits to give birth in the first batch. A female rabbit gives birth to 7 to 8 rabbits in one litter, which adds up to hundreds of umbilical cord blood.
If you still can't practice it, there will be a second batch, a third batch, a fourth batch of rabbits... About every three days, a batch of rabbits will be produced, and the cycle will be monthly.
After practicing with so many rabbits, no matter how stupid or careless the elf is, he can reach the state of "without others, but with familiarity".
With so many assistants, Gretel was able to focus his energy on more valuable areas. For example, directly injecting umbilical cord blood into rabbits and then trying targeted mutagenesis;
For example, throw spells at umbilical cord blood in vitro and try targeted mutagenesis;
For example, try to extract stem cells from umbilical cord blood;
For example, he tried to take unknown cells that had been isolated after multiple centrifugations, and brushed each cell with the spell configuration he designed to see if it could be induced to differentiate into blood cells in a targeted manner;
For example, stem cells still need some environment, such as blood plasma, to differentiate into blood cells. Gretel has to find ways to create an environment for them to work and use his imagination to create seven or eight dozens of versions...
Buzz buzz, buzz buzz...
The centrifuge was spinning vigorously. Grete had a thick record book in his hand, and a pen rolled on the root of the oak stick. He kept recording:
[By the way, the best app for reading and listening to books is Yeguo Reading, www.yeguoyuedu.com a> Install the latest version.】
"In vitro experiment plan No. 1, add 2% hare serum to the culture medium... Targeted mutagenesis model No. 1... Aspirate the suspension after 72 hours, make a smear for observation... A large number of bacteria appeared in the suspension, and the experiment failed..."
"Test tube No. 2... After smear microscopy, no viable cells were found... Repeated three times to confirm that all cells were dead, the experiment failed..."
"Test tube number three..."
"Plan No. 2, 2% hare serum, 0.5% penicillin, 0.5% streptomycin... Directed mutagenesis spell model No. 1..."
"Plan No. 3, 2% hare serum, 0.5% penicillin, 0.5% streptomycin, releases anticoagulant magic... Targeted mutagenesis spell model No. 1..."
"Ahhhhh..."
Grete held his head and screamed. If nothing can be achieved, what is the problem?
Is there a problem with the spell model, or is there a problem with the extraction of hematopoietic stem cells, or is the living environment of hematopoietic stem cells unsuitable?
It is almost inevitable that they are not suitable. Cells are very delicate things, and they are particularly delicate when cultured in vitro.
Gretel did not need to study in depth. He used his superficial physiological and biochemical knowledge to think about it and knew that there needed to be enough oxygen in the culture medium. Of course, the proportion of carbon dioxide must also be appropriate;
Suppressing bacteria is necessary, but problems may arise if too much penicillin and streptomycin are added;
To generate red blood cells, additional iron is necessary, which requires transferrin - then the question arises, how to extract, isolate, or manufacture, human transferrin, or rabbit transferrin, add
In the culture medium?
Ah, by the way, it seems that insulin needs to be added to supply enough sugar to the hematopoietic stem cells... So how should insulin be added?
In vitro experiments are difficult to do, and in vivo experiments are also difficult to do. Not only is it difficult to do, but you also have to slap the table with the elves in the face of surprise, fear, and contempt:
"Learn to draw blood! - Give you one day, everyone must learn to draw blood from rabbits!"
"Learn how to use the syringe correctly! Learn to identify blood vessels! Learn to draw blood from the rabbit's veins correctly and safely! Each rabbit should draw no less than 15 ml of blood and no more than 20 ml each time!"
"Learn smears, microscopic examination, and count blood cells!"
"Learn the magic of blood cell counting!"
The elves cried out loudly. For those who serve nature, it is acceptable to take umbilical cord blood from rabbits. After all, after the rabbit is born, the umbilical cord is useless anyway, and wasting umbilical cord blood is also a waste;
However, grab rabbits one by one, immobilize them, control them with magic, and draw blood from them...
Every whine, every struggle of the body in the hands, warm breath blowing on the arms, wet eyes looking at myself pleadingly...
The stronger the healing ability, the deeper the connection with nature, and the more sensitive the emotions of animals; every bit of fear and pain of experimental animals is like cutting directly into their hearts.
"No...can you please stop torturing them..."
"The purpose of conducting experiments on rabbits is to find a way to replenish blood for soldiers who have lost too much blood! The faster and more accurately you do it, the faster the research progresses, and the less the rabbits will suffer!"
Gretel suppressed their resistance without hesitation:
"Enhancing the recovery ability of warriors is to enhance the combat effectiveness of warriors! - If the warriors cannot withstand it, the plants and animals that will suffer, and the nature that will suffer, will be hundreds, hundreds, thousands of times more than the dozens of animals in the laboratory!"
****
Please subscribe, ask for monthly tickets, ask for recommendation votes, ask for book reviews, ask for all kinds of...