Chapter 466: The biggest difficulty in developing a smallpox vaccine
With the hospital in place and the manpower in place, Gretel rolled up his sleeves and got to work.
The front yard, that is, the outpatient department, is still very popular and empty. Only about ten patients come in every day. The five therapists sitting in the clinic are so busy that one Buddha was born and two Buddhas ascended to heaven. I wish everyone had a cat:
This is not to say that you can relieve irritability by petting the cat. The main thing is that you can throw a ball of yarn to the cat, let it pick it up and tidy it up after it messes up, and you can also find something to do for yourself...
In the backyard, that is, the research department, work is proceeding in an orderly manner. The things that have troubled Leon Carlos for half a year are almost no problem for Grete:
"You try freezing, drying, and freeze-drying. The three methods are applied alternately. The freezing temperature ranges from -20 degrees to -10 degrees, and from 2 degrees to 10 degrees. Each degree is used as a grid."
His face remained calm, and he reported a long list without thinking. Well, how should vaccines be stored? I don’t know, I haven’t learned it, and it’s not in the textbook. This is not the scope of applied medicine. Making vaccines is the work of bioengineering next door...
…
Fortunately, during the epidemic before traveling through time, Gretel had heard about it, and it seemed that there were several storage temperatures. Gretel relied on this fragment of knowledge to talk, and he seemed to be quite able to bluff people.
Well, he is the big boss, so he can just give you direction. If you want to do experiments or something, there will naturally be someone to run the errands for!
Leon Carlos could barely keep up with his speed in brushing the land records. As for how to achieve Gretel's request, it can be done by adjusting the magic circle, but it will burn up the budget...
"If you want to dry, don't use heating. You can use natural drying, dehumidifier, or use magic to drain the moisture." Gretel's instructions continued:
"I remember... there is a magic called [Thirst Touch]?"
"That's right, the first-level magic of the necromantic system." Carlos came to his senses. This magic, through touch, can cause living creatures to quickly lose a large amount of water and fall into a dehydrated state. Although there are quite a few restrictions, the price/performance ratio is not high.
, but it seems just right for vaccine dehydration?
"But, I don't know this magic..."
He was a little ashamed. Although he was a necromantic magician and had advanced to become a formal mage, he was just an academy student. In other words, he had no direct tutor to guide him, and his grandma did not love his uncle.
Some of the most common magics in the necromancy system, such as combating the undead (yes, the introductory magic of the necromancy system is to combat the undead, which can be said to be a model of "I kill myself"), touch of fatigue, touch of cold, ray of weakness,
You can also learn niche magic like [Thirst Touch] in the academy...
"Oh, I'll give you the information later."
Gretel responded nonchalantly. The Horn of Thunder is rich in reserves. Archmage Carlisle threw him countless spell models before, including [Touch of Thirst].
A mere first-level magic, taught to the magician in the mage tower, I don’t think the school would care...
As for freeze-drying, they had done it before when making penicillin and had ready experience. Now they just copy it.
Carlos followed the order and left. Gretel sat alone at the desk, holding her chin in her hands and thinking:
How to verify the effectiveness of the prepared vaccine?
If you switch to a cholera vaccine or a plague vaccine, it would be very simple. A large number of animals can be used for experiments. But the smallpox vaccine will not work. Smallpox is notorious for only infecting humans. What about mice, rabbits, and dogs?
, Pig, you can’t use anything...
Grete began to scratch his scalp with all his strength. After scratching for a while, the oak staff jumped out of his pocket with the [Endless Ink Pen] rolled up and began to write:
Technical route 1: Use cattle as the verification object. 20 freezing temperatures, 10 vaccines per group, storage time of 2 days, 3 days, 4 days, 15 days, 30 days. A total of... 1,000 cows?!
Are there so many cattle around Nevis? How much budget does it take to acquire so many cattle?
Technical route 2: Use primates as verification objects. The same grouping method requires... 1,000 monkeys?
The oak stick trembled. Press the [Endless Ink Pen] and press down, drawing a deep and long mark on this technical route.
Technical route 3: Use humans as verification objects...
Dr. Jenner did the same thing back then, directly vaccinating people with cowpox vaccine, and then vaccinated with variolation vaccine a few months later. Successful variolation vaccination means that cowpox does not produce protection; failure of variolation vaccination means that the cowpox vaccine is successful.
.
The advantage of this route is that the city of Nevis lacks people for everything. If you want to find 1,000 people as experimental subjects, you are absolutely not afraid of not being able to find them; the disadvantage is...
Gretel sadly discovered that he still had some social fear. It was easier for him to sit in the hospital and treat 1,000 patients, but to go out to the street and grab 1,000 normal people and convince them to be experimental subjects for cowpox vaccination.
…
Just thinking about it, I thought, forget it, I might as well kill him.
As a doctor, a doctor in a public tertiary hospital, and an emergency physician in a tertiary hospital, he has never worried about too many patients, not too few. How to attract patients, he has no experience!
Wow, how did Dr. Jenner find so many test subjects back then?
Grete struggled to recall, and sadly discovered that Dr. Jenner seemed to have only done a dozen people. So why did I need 1,000 people? Was there something wrong with my experimental design, or was my requirement too high?
Hey, I was too hasty before. All the apprentices and nurses have been vaccinated, and there are no experimental subjects left...
Gretel hugged his head, groaned, and hit his forehead on the desk. A bang sound startled Anivia who came in through the door:
"Sir, what's wrong with you?"
"Who should I inoculate with cowpox..." Gretel put his forehead on the edge of the table and hummed. Anivea came forward holding the tray, took a look at the manuscripts on the table, and laughed:
"A thousand people? Sir, it's easy to get a thousand people. In which month has our treatment center at the port not been able to get a thousand patients?"
Gretel stood up.
yes!
Make the treatment center famous!
Let the people around you feel that this is a reliable place!
Grete grabbed the pen from the root of the oak stick and began to write. Once his ideas started to open up, the ways to find people became more colorful:
1. Build the reputation of the treatment center and attract more patients. This is the right path. If you run it well, it has unlimited potential;
2. Go to the surrounding areas to send doctors to the countryside, and vaccinate people on the way. It must be said that he is wearing a linen robe, holding an oak staff, and riding an Apa. He is still full of momentum and is easy to believe. Find 20 experimental subjects in one village
, visit 50 villages, and the task is completed;
3. Talk to the city hall and ask prisoners to do experiments. The city hall may not necessarily buy it. It may first communicate with the public health department, or use the teacher's banner;
4. Ask the surrounding lords for help. They will lead the people and provide vaccines themselves...
Although the verification of vaccinia vaccine requires a thousand people, these people do not have to come at the same time: considering that later vaccination with variola requires isolation and observation, in fact, only 20 to 40 people are needed in each batch. If there are more, the hospital will not accept
Even my diagnostic ability cannot cope with it.
Calculating it this way, the pressure is actually not great.
"Annivea! Let's go to the front for a consultation!"
However, Grete's joining did not make the situation better at first. For the poor people who came to their homes, experienced therapists would give some medicine for mild cases and throw in treatments for severe cases, and the problem would usually be solved within ten minutes.
Only Grete, ten minutes is not enough for him to check:
"Sir, what is your name?"
"age?"
"What's wrong with you?"
"Stand up and let me take a look... Hey, take off your coat and take off all those metal accessories!"
"Okay, lie down, lift up your clothes, and let me take a look..."
Inspection, questioning, auscultation, palpation, X-ray, B-ultrasound, and electrocardiogram. The time to collect the patient's condition is three times longer than the time to treat. In this way, the final answer is probably still:
"Sir, please take this packet of medicine..."
"Sir, let me give you a healing technique..."
Just, it's a huge waste of time.
And they even steal patients!
The priests who worked with him looked sideways. Mr. Gretel Nordmark, although you are our employer, can you stop taking our jobs?
We also need to accumulate treatment experience as we advance!
Because the amount of treatment here is too small, we have divided the work, and each person only comes for half a day!
Mark Saren, the third-level priest of the Ares Temple, rolled up his sleeves, put the treatment table on his shoulders, and silently moved it to the gate.
Hannah, the second-level priest of the Order of the God of Nature, followed immediately. Without asking for help, she urged the plant to roll up the two table legs, then raised one edge of the table herself, and moved the treatment table to the other side of the door.
Gretel: "..."
As for that? As for doing this to me? Didn’t I just spend more time checking? I didn’t leave you to work overtime!
Huh! If you have the ability, just keep on grabbing! If you have the ability, don’t run into incurable diseases that you can’t handle!
Grete was angrily drawing circles in her heart. However, the incurable symptoms soon appeared. Mark came over and called him with a face full of embarrassment:
"Master Nordmark, would you like to come over and take a look? I have treated this patient more than once and he keeps relapsing!"
Grete got up and went out. When the visitor saw him, he put on a smile, his chubby face spread out to both sides, and his eyes narrowed until there was only a slit left:
"Pastor Nordmark! Do you remember me?"
...To be honest, the face is a bit familiar, but I can’t remember the name. Grete swept from top to bottom, from bottom to top, and paused at his obviously enlarged finger joints, and suddenly realized:
"Oh...you are the one...I treated gout for you! How are you doing recently? Do your fingers and toes still hurt? Do you still eat sea fish and wine?"
"I don't dare to eat. I don't dare to eat." The businessman nodded and bowed with a smile on his face. He helped the white-haired old man walk into Grete's consulting room and sat down across from his table:
"My father coughed up blood again... He went to see more than one priest, but he was cured at the same time, but relapsed two months later... Pastor Nordmark, can you take a look?"
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