Chapter 77 Gene Spray
Jiang Yan's number on the magic forum is No. 2. He has many questions and wants to ask Merlin directly on the forum.
Merlin, as the manager behind the forum and the discoverer of magical civilization, Jiang Yan hopes to get answers from Merlin.
"Mr. Jiang, how is the situation of the Magic Forum today? We are still reporting regularly, and I will return the information to the headquarters when the time comes." The staff member asked after entering the office.
"I can only see my number. The number has always been 2 and has not changed. I cannot see the total number."
"There is still only Merlin's single post on the forum, and no one else has posted anything."
"I still haven't figured out how to post. Today's situation is the same as usual."
"The abilities brought by the Demonic Brain have also not changed, and they still maintain a vague perception of the seeds."
"Now all abilities are in a black box state. They can be used but the principles cannot be explained."
After Jiang Yan gave a brief report, the staff continued to ask:
"Sir, after reading "Zhou Yi Shen Tong Qi" and "Enlightenment Chapter", did your demon brain react to these contents? Or did you have any insights?"
"Zhou Yi Shen Tong Qi" and "Wu Zhen Pian" are both Taoist works on inner alchemy practice. The Bureau of Anomaly Investigation specially found the ancient and handed down copies for Jiang Yan to study.
"I don't have any understanding. These contents and magic do not belong to the same system at all. I think the Taoism methods passed down from ancient times are not the same thing as the magic brain."
Due to the emergence of magic, the Bureau of Anomaly Investigation not only collected various ancient collections handed down from ancient times in China, but also sent people abroad to collect magic-related information.
Some ancient magic-related cultural relics have been sold for sky-high prices, especially those unearthed in England.
It is speculated that Merlin is from England, and the name Merlin itself is also a magician in English myths and legends, so England's magical relics are particularly favored by those in the know.
After completing the day's routine question and answer session, Jiang Yan continued to think about it, which he found very strange and even a bit funny.
Merlin said in the post that the Magic Forum was created for everyone to communicate. It was claimed to be to let everyone realize the value of thinking, and it was a platform for communication and value display, but he did not see the exchange or communication.
It's like the group leader said everyone is welcome to speak, and then banned all members.
Jiang Yan was a little puzzled because the news he received was that he was not the only one in the world to have obtained the magic brain. China speculated that there was at least one magic coin in the world where Enceladus was registered.
At present, there are two speculations in Huaguo. Either the other magic coin has not been used yet. It is possible to speak on the magic forum, but Jiang Yan has not found a way yet.
Another guess is that another magic coin has been used. The reason why the magic forum cannot post so far is that certain conditions have not been met.
At present, regarding magic, America is very close to England, while China has chosen to share limited information with Russia. We currently do not have a communication mechanism on this topic.
...
"Teacher Jiang, we don't actually need to select seeds in this way. It will be difficult for the seeds selected in this way to achieve the desired effect, and it will not be able to form a sufficient variable control group."
"The seeds cultivated in this way have different mutation points. It is difficult to draw conclusions through controlled experiments. We cannot publish a paper like this." After another week of breeding time, Dong Wenxu reminded again.
He continued:
"If you want to cultivate genetically mutated seeds on a large scale and feel that the current conventional methods are too inefficient, we can use carbon dot spray for gene editing."
"Carbon dots formed from spherical natural materials with nanoparticles smaller than 10nm can serve as rapid vectors to carry plasmids into mature plant cells."
"This results in instantaneous plant transformation of species in many important crops, with no negative impact on photosynthesis or growth."
"Introduction of wheat's GFP,A expression gene fragment via foliar application (spray) of plasmid-coated carbon dots and, in the case of Cas9, genome editing in the SPO11 gene."
"This spray-based gene editing protocol is simple, cheap, fast, and applicable to a variety of crops."
"And it is more targeted, making it easier to conduct subsequent controlled experiments."
Nowadays, in the subdivision field of biology, breeding, the more cutting-edge research methods adopt directed genetic differentiation to study the differences between assumptions and actual situations.
Like the mutation rate that confers herbicide resistance in natural plant populations and examine whether the mutation rate increases when plants are stressed by sublethal herbicide exposure.
The scientists used the cereal amaranth and its resistance to acetolactate synthase (ALS)-inhibiting herbicides as a model system for discovering spontaneous herbicide-resistant mutants, screening a total of nearly 80 million plants.
As for Jiang Yan's research method, not to mention how far behind the most advanced research method is, it belongs to the research method of Shen Nong who tasted a hundred herbs in ancient times.
Jiang Yan knew that something needed to be done to stop Dong Wenxu from thinking too much: "This method was developed by Thomas from Bristol and Keith from the School of Chemistry in 2019."
"I'm more familiar with this than you are. The problem with this method is that it doesn't guarantee that the gene editing you want will happen."
"You cannot guarantee that the plant will be transformed after spraying the rapid vector. In real experiments, there is a chance of this happening. How do you select seeds with real genes that induce differentiation?"
"Shouldn't we still go through conventional experiments? This method has no advantages over the current mainstream experimental methods. It looks faster, but it's only how fast you feel."
"The screening methods of this method are more troublesome than conventional ones."
"If this method was really as superior as what Thomas wrote in his article, you would have seen it promoted on a large scale at the Institute of Agricultural Sciences."
"I know you don't believe that I can screen out better seeds based on my feelings. You think that even if I can screen out better seeds, I won't be able to publish papers with sufficient impact factors without reference experiments."
Dong Wenxu was dumbfounded. He had only seen this method in articles and had never actually practiced it, let alone pointed out its disadvantages.
What shocked him even more was that he had indeed read this article and was deeply impressed by it. He mentioned it in order to change Jiang Yan's research methods little by little, but he didn't expect that the other party would blurt out the ins and outs of this paper.
Even the author of the paper, the university where it is located, and when it was published are all clear.
Chapter completed!