"In agriculture, a paper is just a summary of your work. The essence of evaluating the quality of your work is the positive impact your research results have brought to agriculture. Publishing a paper is only an essential incidental impact."

"I have no way to convince you to believe my method now. The maturity period of cabbage is about forty days. If you take good care of the crops carefully, the final results will prove that I am right."

Dong Wenxu didn't know where Jiang Yan's confidence came from. He just hoped that his work during this period was not in vain.

However, during the planting process, Dong Wenxu could enjoy it. He sometimes thought that the Chinese people's farming genes were too strong.

Farming is an instinct deeply rooted in the genes of Chinese people. Jiang Yan continued to invest in agricultural research after obtaining the standard magic brain.

Simons of American is studying the epidemic. Regardless of whether the new coronavirus is caused by nature or Fort Detrick, one thing is certain that American is very worried about the epidemic.

They have been crazy about over-issuance of currency in the past year. If the epidemic is not resolved for a long time, it will become more serious and the global supply chain cannot return to normal. America's economy will be in a greater crisis.

Jiang Yan can sense life, so Simmons also has this ability, and the characteristics given by the standard magic brain are the same.

Simmons has joined Pfizer's new coronavirus drug research and development team, and Amerika hopes that he can work magic.

Remdesivir, which was previously trumpeted by the FDA and the domestic media, has completely failed as a specific drug for COVID-19.

The WHO publicly issued a statement at the end of last year saying that no matter how severe the condition of COVID-19 patients, it is not recommended to use the antiviral drug remdesivir for treatment.

At that time, domestic public intellectuals even nicknamed Remdesivir "the hope of the people".

The technical route of nucleoside analogs has almost failed.

The cost of research and development of new drugs lies here. A large amount of research costs and clinical trial costs are spent, but in the end the results are not as good as expected, and these costs are wasted.

Sometimes, the greater the investment, the greater the disappointment. It is precisely because of the high risks of scientific research investment that companies like Kechuang Biotech, which holds endorphins, can enjoy such a high valuation.

The biggest difficulty with RNA epidemic viruses like COVID-19 is that they mutate too quickly, and strong mutations often occur accidentally. After the mutation, the shape of the virus spike protein is changed, causing the virus to be immune to the effects of vaccines.

"To a certain extent, vaccines cannot completely immunize against COVID-19. Only by relying on specific drugs can we minimize the adverse effects of COVID-19."

"You can even use the new crown to make the biggest piece of the cake."

Due to the epidemic, vaccine manufacturers, testing reagent manufacturers, and even medical device manufacturers all made a fortune during the epidemic.

If Pfizer develops a specific drug, they will steal all the cakes from other manufacturers.

"Sorry, I can't tell which method is better, but judging from the current twelve kinds of mice injected with antiviral compounds, I think this one's life status is better."

In front of Simmons were twelve mice, each injected with different proteins.

Since the virus emerged, researchers have been working hard to find drug targets for the new coronavirus.

There are two main strategies to control the new coronavirus. One is to discover inhibitors that prevent the virus from entering host cells, and the other is to discover bioactive compounds that prevent viral replication and transcription.

Pfizer researchers identified a dozen proteins that were identified as potential targets.

They can be roughly divided into two categories: the first is structural proteins, including spike proteins, envelope small membrane proteins, membrane proteins, nucleocapsid proteins and another category of proteins called non-structural proteins.

Among them, non-structural proteins mainly include protease (M pro), papain, nsp13: helisp12: R RNA polymerase, nsp14: N-terminal exoribonud 7 methyltransferase, nsp15: uridylate-specific endoribonuclease, nsp16

:2'-O-methyltransferase and nsp10.

The new coronavirus is responsible for replicating and packaging the viral nuclear material into the capsid protein shell.

Blocking any of these proteins causes a stall in viral replication or packaging of its RNA into a protein coat.

Of the total 12 proteins identified as targets for controlling CoV; 3CL pro was identified as one of the most important targets due to its critical role in mediating viral replication and transcription.

3CL pro is a chymotrypsin-like cysteine ​​protease (~33 kDa) that is similarly known as the major protease due to its dominant role in processing replicase polyproteins and genes.

Although 3CL pro is a dimer, which provides the substrate binding cleft, the protease exerts its proteolytic activity through nucleophilic attack directed by cysteine ​​thiols in its Cys-His bimolecule.

The activity of 3CL pro during the viral life cycle is lacking evidence from its human analogues.

Therefore, Pfizer researchers only believe that 3CL pro may be used as a potential main drug target to bind and inhibit the replication activity of the new coronavirus.

They need Simmons to judge from his perspective.

There is a high degree of similarity between the structure of 3CL pro and the 3CL pro of different new coronavirus mutants. Inhibitors previously designed for MERS or SARS can also be effective against the current epidemic of new coronavirus.

At the same time, an inhibitor called N2, which was previously designed to inhibit 3ERS-CoV, was also involved in testing against the new coronavirus this time.

Some researchers have proposed targeting 3CL pro, among which the efficacy of available drugs such as ritonavir, emetine, hesperidin, lopinavir and indinavir have shown the potential to inhibit 3CL pro.

The phytochemicals of green tea have also been evaluated in depth to identify phytochemicals with the potential to inhibit 3CL pro.

In addition, neem phytochemicals were also evaluated against the target proteins of SARS-CoV-2, namely membrane protein (M) and envelope protein (E), with Nimo, 24-Methyleaa-Amyrin and Nimbolin A being used by Pfizer.

Researchers injected it into mice.

Simmons touched the mice one by one. I don’t know whether it was his psychological effect or it was really the case. He thought that touching the mice with his own hands would help him make a better judgment.

"I think this is just the best guinea pig here."

The researchers next to him quickly disinfected him, and another researcher asked: "This mouse was injected with a protease inhibitor."

"We developed protease inhibitors based on five popular M pro variants in the new coronavirus lineage, including G15S, T21I, L89F, K90R and L205V."
Chapter completed!