Chapter 52: The occult version of 'Chromatography'
"Uh..."
Xilan and Qiong followed the direction of Fan Ning's finger and looked towards the end of the table.
The sink, washbasin, brush, alcohol, washing powder, steam oven...the whole set of utensils are neatly placed there.
"Okay, it's not impossible." Qiong curled her lips, "But I'm mainly curious about what you want to do. Maybe you don't believe it, but distillation, crystallization, centrifugation and other methods are really inseparable from these extraordinary components.
In very few cases, the knowledgeable people of high rank and above rely on their strong inspiration control and use certain means to pull out the chaotic transmutation products, and this is still incomplete separation and purification."
Fanning has already started his operation.
"Stay away first. You are not wearing a mask. It will be difficult to expel this stuff after it is inhaled into your lungs."
He cut open the silica gel powder packaging bag, poured half of the volume into a large glass with a capacity of more than one liter, and then poured finely ground fusilized particles and burnt ash into it according to the volume ratio of 5% and 2% respectively.
The black bones of the scum have light spots on the glands.
Flint particles are a kind of migrating substance. In certain dreamland areas where the light and shadow are close to noon, those who know can collect a heavy and shining crystal on the surface of the dust. When you slide your fingers through it, you can feel the affinity of inspiration for it.
The black light spot is a kind of "trace spirit" in the migration. This kind of material is considered to be "the leftovers of the glow and the memories of history that are constantly drifting downward." Although there are living things in the migration,
characteristics, but they are far inferior to 'moving creatures' and cannot affect the waking world.
The "skin of the end", the production carrier of common curse seals, also comes from a plant-like trace spirit in the migration.
Dust is floating in the air, and after mixing, the silica gel has the ability to adsorb extraordinary substances similar to ordinary substances.
Fan Ning picked up a large Erlenmeyer flask with both hands and raised it to his eyes for observation.
"What is this?" Sheeran was very curious.
"Water, and 8% industrial cellulose that has been roughly carboxymethylated." Fan Ning said, "You can understand it as a thickening or binder that is not easy to use and is quite expensive."
"Are you a transfer from St. Lenia Polytechnic?" The two girls touched their heads in confusion.
"It should be ready after letting it sit for two days." Fan Ning pulled out the stopper, poured the supernatant inside into another glass, then added the processed silica gel powder, and stirred it into a large ball of light gray with a little transparent glitter.
Textured viscous slurry.
Then he took out a 10x10 cm glass plate, scooped out a large spoonful of slurry with a spoon and placed it in the middle, tilting it left and right to allow the slurry to flow evenly on the surface under the action of gravity.
"What does this have to do with isolating extraordinary components? Caroln, are you making pastries? Is this edible?"
Seeing Fan Ning put the square glass plates coated with glitter slurry into the oven one after another, Qiong couldn't help but ask.
"Aren't you full yet?" Fan Ning closed the oven door, turned on the steam heater, then returned to the table, reached out and pulled down a small handful of cotton from nearby.
On the steel bracket in front of the wall, a long glass column has been fixed with clips. Its upper and lower length is nearly one meter, but its diameter is only three centimeters. It has a frosted opening at the top and a glass bolt at the bottom.
Fan Ning threw the cotton in, gently pressed it against the bottom outlet with a long glass rod, and then spread a layer of washed river sand on top.
He unscrewed another brown bottle, poured the gray clear liquid into another part of the silica gel powder, and stirred it into a slurry.
There is a smell slightly like something rotten floating in the air.
"What is this?" Sheeran asked curiously.
"Blight Moth." Qiong explained, "a special solvent with a low boiling point that he screened before. It is made from the migrating trace spirit 'Blight Moth' and some light petroleum components. It has relatively poor solubility.
It is narrower and has weaker background interference with spiritual perception. It has some applications in the crystallization of extraordinary substances."
"But I still don't understand." She couldn't help but ask again, "Caron, why don't you try to dissolve the sample with methane and mix it with silica gel? These two things are incompatible.
Rong, it's just a ball of sticky slurry. Also, what is the purpose of making this pillar of yours so thin and long?"
"I'm loading 'column chromatography'." Fan Ning raised the large glass of slurry, poured it into the column with a glass rod, and then used a dropper to absorb a few pieces of methane, rotating it at the top of the column and squeezing it out, removing the remaining residue on the wall.
Pour the silicone powder down and finally plug the top.
The mini steam blower connected to the rubber tube began to buzz, compacting the silica gel slurry in the column downwards.
Methane flowed out from below, and he caught it with a cup and poured it into the top again. After repeated operations several times, a large section of silica gel column with a contradictory sense of darkness and sparkle appeared in the glass, and there was still a silica gel column at the upper border.
A few centimeters deep of fentanyl liquid seals the seal.
"Column chromatography? Did you invent this? This column...can separate extraordinary components?" Joan looked closer in disbelief.
"You'll find out later." Fan Ning smiled mysteriously.
Fan Ning filled several such pillars, some thick and some thin, in turn. After plugging them, they clamped them in the air with clamps and lined them up on the steel supports.
A few people wandered around casually for a while. In the evening, Fan Ning opened the oven door and took out the tray. The silica gel slurry on the square glass had become dry and smooth.
He took out a glass cutter, drew straight lines two centimeters apart on the back, and then "bang bang" broke it into glass strips.
Before Qiong could ask, he took the initiative to explain: "This is thin layer chromatography... I will first take an extremely small sample, use it to detect the distribution of special components in it, and then formulate a separation strategy and use column chromatography to separate.
"
Whether it is column chromatography or thin layer chromatography, their separation principles are the same:
Under the erosion of the mobile phase, different substances move through the stationary phase at different speeds - strictly speaking, the moving speed here is the cycle process speed of "adsorption-desorption-re-adsorption-re-desorption".
In Fan Ning's previous life, the common stationary phase was silica gel, and the mobile phase was organic solvents such as petroleum ether/ethyl acetate/dichloromethane. In this world where mysticism is popular, the stationary phase he chose was treated with extraordinary substances.
Silica gel, the mobile phase is the cyclane he selected, as well as the coelite lymph fluid and silverfish instar passivation ester that will be used next.
After preparing both column chromatography and thin layer chromatography, he finally started processing the samples.
Fan Ning was still a little excited and uneasy at the moment. Separating the extraordinary components was only a small part of the meaning. More importantly, if the principle of chromatography could be reproduced in this world, those so-called "waste formulas" in the future would be
After the lethal by-products of chaotic transformation are separated, they will become high-level spiritual elixirs with sky-high prices!
He put each of the three pigments into the separatory funnel one by one, first added water and stirred it into a turbid suspension, then poured in an equal volume of coelite lymph, shook it, let it stand, and layered it.
This is a special solvent with strong and wide solubility. At this time, most of the mixed special components in the pigment enter this layer. Under the spiritual perception, the dilute solution presents chaotic light and shadow with mottled colors.
Open the stopper, separate the liquid, add it again, shake it and let it stand for layering...
After looking at it for so long, Qiong finally found something she could understand, so she turned to Xilan and said: "...His extraction operation is more standard than mine, and he is the most skilled among the musicians I have ever seen...I now feel that he
He must have secretly gone to the Chemistry Department of the Polytechnic Institute to study..."
The sample turned into a raw solution. Fan Ning took out several micro-sample tubes the size of the tip of his little finger, labeled them, and sealed them one by one for storage.
Then, he picked up the measuring cylinder.
"Configuration of the mobile phase...try to first try a ratio of pyridane and coelite lymph fluid of 5:1, and then add five drops of silverfish instar passivation ester."
After Fan Ning finished measuring, he poured the mixed solvent into a cylindrical glass bottle. The amount he used was very small, and the depth of the liquid in the bottle was only half a centimeter.
This bottle is where TLC will be developed later.
He took out a piece of thin layer chromatography that had been divided into rectangles, used a pencil to draw a straight line one centimeter from the bottom of the silicone surface, marked three points on the straight line, and wrote the small letters 1, 2, and 3.
Use a capillary glass tube to gently absorb the original pigment solution from the watch factory, the original pigment solution from the almshouse (yellow branch), and the original pigment solution from the underground building, and click on the positions 1, 2, and 3 on the straight line in sequence.
Finally, he used tweezers to pick up the thin layer chromatography glass piece, put it into the cylindrical bottle, so that the straight line of the pencil was above the liquid level, and closed the bottle cap.
Just like water soaking the paper surface, the mixed extraordinary solvent began to creep up on the silicone surface. Visibly to the naked eye, a darker area was spreading from bottom to top.
A minute later, when the dark side was almost reaching the top of the glass plate, Fan Ning pinched it out.
The low boiling point extraordinary solvent quickly evaporated in the air, and the dark side disappeared. The silica gel on this glass plate looked no different from before. Even with Fan Ning's spiritual sense, he could barely see the heterogeneity on it.
light and shadow.
Because the concentration is too dilute.
"What happened?" Joan and Sheeran looked confused, but when Fan Ning moved the glass plate to the gem altar that was already in operation, they saw a surprising scene through the crystal ball: three "runways"
, the top is split and iterated stripes of light and shadow, and there are golden spots in the middle of No. 2 and No. 3!
As expected, Fan Ning expected that although his spiritual sense could not be distinguished due to the low concentration of the extraordinary components, he could use the crystal ball + altar to strengthen his spiritual sense.
Just like the method used to observe thin layer chromatography in previous lives, there is ultraviolet lamp or iodine vapor...
In this world of mysticism, the way to observe extraordinary matter is spiritual sense + secret ritual. Of course, if you want to further characterize it, you may still have to rely on the accumulation of hidden knowledge of knowledgeable people to judge after separation - it may be difficult to move matter.
Understood as a molecule or pure substance.
For the purpose of "revitalizing waste spirit formula", it is enough to be able to observe and separate them.
Joan is also a knowledgeable person who is quite talented in elixir science, and she immediately interpreted this phenomenon.
Sample No. 1: The pigment from the watch factory only contains a single extraordinary component of a certain "derivative" phase.
Samples No. 2 and 3: Workhouse and underground building pigments. In addition to the "derivative" phase component, there is also a certain "candle" phase component that was separated by Fan Ning!
"Caron, what is your invention called... thin layer chromatography? I didn't expect this method to be really useful!" Looking at the thoughtful Fan Ning, Joan's tone was very excited.
"There are very few cases of successful separation and mixing of extraordinary substances. I didn't expect that you would succeed in your first practice of elixir science. The cost is very low and the time is very short. You are really lucky!"
"Luck?" Fan Ning smiled softly, "You will know right away, my method can be reproduced in most situations."
"Change the ratio of pyridine to coelomyzoid lymph to 15:1, and add two drops of silverfish inactivation ester." He measured the new mobile phase ratio and poured it into a cylindrical glass bottle.
On the second thin layer chromatogram, he drew a straight line, wrote down the numbers, and used the capillary glass tube to draw the original solution again.
At this time, Fan Ning wrote a number 4: the elixir that principals take.
Immerse the lower end of the straight line in the cylindrical bottle in the same manner, and pinch it out when the special solvent is about to climb to the top.
The next second, Joan stared at the crystal ball in the altar with a look of shock on her face.
Sample No. 1: The paint from the watch factory is still "evolved" at the front end, but it doesn't run that far.
Samples No. 2 and 3: For the workhouse and underground building pigments, the "evolution" also did not run that far, while the "candle" phase component basically did not "move" and still stayed at the starting position.
What is even more surprising is Sample No. 4: the elixir taken by the principal. There are three kinds of light and shadow in it. The top is "Yan", followed by the pink "pool" and the green "cocoon", but they are all before
On top of the "candle".
There are actually four extraordinary substances that appear independently on this so-called "thin layer chromatography"!
Joan shouted out in excitement. Fortunately, the third floor was empty and the building's sound insulation effect was also very good.
Fan Ning was also vaguely excited at this moment, because he found that his conjecture was correct, and the principle of chromatography is indeed applicable in this mystical world.
The only difference is that the flow speed of ordinary substances in the chromatogram depends on their polarity, or the degree of molecular symmetry. In a forward column environment, the more symmetrical the polarity, the faster the flow, and conversely, the more asymmetrical the polarity, the smaller the polarity.
The greater the polarity, the slower the flow. By adjusting the mixing ratio between several highly polar and weakly polar solvents, you can control their unfolding and keep them in the most appropriate position.
As for extraordinary substances... Fan Ning initially discovered that their flow speed seems to be related to the overall ordering of the seven phases! Under the current extraordinary solvent configuration, the fastest unfolding is "Yan" and the slowest is "Candle"...
His idea is also based on the solubility properties of extraordinary solvents, using a combination of high and low performance solvents, and controlling the expansion by controlling the proportion...
After summarizing the preliminary rules of mysticism in his mind, Fan Ning's brows gradually wrinkled.
The first is the issue of "candles"... Since what he chopped off with a crowbar in the underground building happened to be a piece of yellow paint, the Langevni brand paint he chose first this time was also yellow. Looking at it now, they all have "
"candle" ingredients.
Furthermore…
His eyes stared at the several iterative "derivative" light and shadows on the TLC silica gel.
Both pigments and elixirs are "derived"? Is this a coincidence or...?
Joan was obviously aware of this problem: "...Carolen, the different raw liquid 'runways' and 'evolved' phases of light and shadow you have here all climb to the same height...Does this mean that they are the same extraordinary substance?"
"It's extremely possible, but a more rigorous verification method is needed."
In order to make the "derivatization" climb lower, Fan Ning changed the mobile phase combination with lower solubility - pyridane: coelomydae lymph fluid = 30:1, and added a drop of beetle instar passivation ester.
He took out a third TLC glass plate, and this time he ordered an additional mixing point for several samples based on the previous one.
Starting to climb again, several people discovered that at this time, 'Zhu' and 'Cocoon' were almost not moving at all, and they were all mixed up at the starting point. 'Chi' went up a little, and 'Yan' was in the lower middle position. Moreover, all
The height of the "evolution" in the runway is still exactly the same!
"The elixir that the principals consumed...contained an extremely small amount of the extraordinary radioactive component found in the pigments of the Golden Langnia Watch Factory!?!?" Fan Ning was dumbfounded.
Elixir maker and Dean Graham, professor of the Department of Chemistry at the Polytechnic Institute?…
Pigment supplier for watch factory, Relatim Chemical Trading Co., Ltd.?…
The situation in the imperial capital…
Penetration of the School of Reconciliation…
The Special Patrol Office has an elusive position...
The investigator who went crazy because of the "fountain in the painting"...
Many pieces of information immediately appeared in Fan Ning's mind.
Sheeran's face showed deep concern: "...So, how long have Principal Sternikai and Vice Principal Huxley been taking this elixir?"
Fan Ning tried to calculate the time that had passed since the premiere of "Symphony No. 1".
Today is already the night before leaving for the performance in the Imperial Capital!
"Maybe...you've been eating it for almost two months?"