Fan Ning's mind quickly flashed through the memories of his several meetings with the two principals during this period.
It seems quite normal.
The extraordinary concentration of active ingredients in the "cocoon" and "pool" phases of this continuously taken elixir is already very low in a single dose. In comparison, the "derived" phase components that are suspected of being radioactive substances
It's even thinner.
The "derivation" in the watch factory's pigment is also very thin, but there is no interference from other extraordinary components. If this healing elixir had not been separated by thin layer chromatography, the spiritual sense would have looked like a blooming and churning dark green.
Nothing unusual was noticed.
If there is any change, it may be slow and invisible.
Fan Ning quickly opened the drawer, wrote a few words on the note paper, stuffed it into the envelope, and went downstairs to ask the clerical staff to deliver it to Roy first.
Although I didn't know the specific situation, I quickly suggested that they stop taking the medicine and put Dean Graham under control.
Furthermore, the "derived" phase components in the watch factory's paint are the same as the paint in the Llangevny workhouse and the paint scraped from the underground building!
The authorities' investigation results show that labor symptoms have nothing to do with the production process?
The investigation results of the Special Inspection Department show that the person in charge of Jinlangnia Machinery Factory subjectively did not know that there was a secret organization infiltrating and lurking? Therefore, in accordance with the principle of "ignorant people have no obligation to guard against secrets", he was exempted from the responsibility of the mysterious side?
"The authorities and the Special Patrol Office are giving this fucking bullshit," Fan Ning sneered in his heart.
"Do you want the Llangevni pigment factory in Carlow now?" Sheeran asked after Fanning re-entered the door.
"It's getting dark." Fan Ning shook his head, "Tomorrow we have to pack up our things and rush to the imperial capital early tomorrow. We can only ask the Guidance School to dispatch the police and security bureau tomorrow to seal the almshouse, and the factory owner Stanley.
Catch him and talk about it later.”
"And Julius." Joan reminded.
Fan Ning had told them before that Julius seemed to have become a wise person.
"Her? Come with me to the Imperial Capital for now." Fan Ning, wearing a white coat, paced back and forth in the elixir room, thinking.
"Legal speaking, the person in charge of the workhouse and factory is only Stanley, not her. The only way to deal with her is to use force or report to the Special Patrol Office for suspected violation of taboos. This seems to be impossible for us.
Bringing clear benefits, or tracing clues to the Experience Officer 'Erov'
"And more importantly, where are we going to find another concertmaster the night before we leave? "Violin Concerto in E minor" Sheeran can't wear two hats."
"Should we inform the school about her situation first?" Qiong asked.
"I just wrote it all together," Fan Ning said, "but I really don't know who to report it to. You Bologna School are almost sifting through it. I sent the letter to Roy and let her make her own judgment."
"In this trip to the imperial capital, let's keep a close eye on Julius. This girl takes her status as a prima donna and her artistic reputation very seriously. At least we all have the same goal before the performance ends. We will wait for the results of the concert to settle, and for the record to be released.
The number of banknotes I want is sold in the pre-sale. Let's meet the first-level wise people like Ji Xingxing. I can bake ten of them with one 'Fire Sun Guidance'... During the sojourn, her life and sleep are under my control.
I want to see if she can help me find any clues in the imperial capital."
He took a deep breath and sat down again: "What we need to do now is to completely separate the extraordinary components in the sample. When the time comes, the evidence will be packed and slapped directly on their faces... Only pure extraordinary substances can be easily studied.
Where are the mysterious properties?”
After this key point is resolved, all that is left is for Ms. Hamilton to complete the closed loop of evidence on on-site epidemiology and labor occupational injury damage assessment, and then rely on her and Monroe's rich legal experience to raise the upper limit of compensation.
Many things are just the tip of the iceberg. If this case can be successful, it will not only be meaningful to help this group of victim families, but also serve as a basis for protecting the rights and interests of workers who are potentially victims of the same type now or in the future.
Joan already had a strong interest in the science of elixirs. At this moment, she took Fan Ning's unfolded TLC plate and repeatedly took it behind the altar crystal ball to observe - this magical phenomenon was enough to keep her going for a long time.
The novelty of time.
"Caron, I didn't expect that it can really separate extraordinary components, but I think the amount seems to be too small." After looking at it for a while, she raised her question again, "This is what you just used a capillary glass tube to separate on the silica gel surface."
Did you click on it? This is probably less than one part in tens of thousands. Even if you click a few more times, we will probably have to work non-stop for several months to separate all the components in the sample.
…”
"As I said before, thin layer chromatography is just a detection method." Fan Ning said with a smile, "And for real separation... what we need to use is it..."
"I have special separation skills." Fan Ning pointed to the row of installed column chromatographs clamped on steel supports.
Qiong was looking thoughtfully at these slender glass columns when she suddenly saw Fan Ning take out a large flask and mix all the extracts of the three pigments together, and then he also poured out the two elixirs.
Got in.
"Caron, what are you doing!?" Both girls exclaimed in complete confusion.
"Separation is so difficult, how do you get them together?...You got four samples at once, how do you separate them??"
"Yeah...you have a special mixing technique, right?"
"Extraordinary solvents are quite expensive." Fan Ning explained seriously, "If you divide it into one, it is divided, and if you divide it into four, it is divided. Why not do it together? Our time is also quite precious."
"Is the method you invented so stable and violent?" Joan's eyes widened, "So you can separate all the components no matter how complex they are?"
"Theoretically, this is true. By gradually adjusting the solvent ratio of the mobile phase, I can precipitate all the extraordinary components from "derivatization" to "candle"... If the components are too close to each other on the spectrum, for example, I guess the two phases are the same.
Different types of extraordinary substances will be similar, which requires a longer chromatographic column, a wider cross-sectional area or a gentler elution pressure..."
"Of course, these extraordinary substances cannot transmute each other..." Fan Ning pointed to the pile of mini sample tubes with the glass rod in his hand, "I just tried spotting the mixed solution, and it's okay. This system is still relatively good."
It’s simple and has common components, so don’t waste it…”
Especially the "derived" component, the concentration is extremely thin, and only the light and shadow brought by the underground building are slightly stronger. If they are not put together, Fan Ning is afraid that the size of the final precipitation will not even be smaller than a fingernail...
"Watch it, I'll explain it while I'm operating it. When I need to refine some high-level spiritual elixir in the future, how about you also help me with the pillars? We can make money together?" Fan Ning looked at them playfully and dug in his hands.
Pour two spoons of specially processed silica gel powder into the mixed solution.
He connected the mixed stock solution flask mixed with silica gel to his modified "rotary evaporator", then opened the rotor and started the steam heat source.
"The steamed coelomycete lymph has to be condensed, and special solvents are quite expensive... Remember, while maintaining full adsorption, use as little silica gel as possible. The thinner the silica gel is when it is poured into the column, the better the separation effect will be later.
If it is too thick, there may be a tailing phenomenon where the back catches up with the front... I use the thickest chromatographic column here because it makes it thinner..."
After the solvent evaporates, all these extraordinary components in the bottle are adsorbed in the dry silica gel powder, creating a faint light of mixed colors under the spiritual sense.
Fan Ning took out the sample paper, folded it into a funnel shape and placed it on the mouth of the column, then poured the colorful silica gel powder into the chromatographic column.
"This is a 30:1 volume ratio of pyroxane: coelomycoid lymph fluid, and a drop of the solvent of the inactivated ester of silverfish instars is added. We start with the ratio with the worst solubility and gradually increase the ratio. We must not
Too much force... Judging from our previous thin layer chromatography detection results, if the proportion of coelomycete lymph fluid is too high at the beginning, the extraordinary components of several phases will be flushed out together..."
Sheeran felt dizzy after hearing this, but Joan seemed to be very interested. She put on a small white mask, sat next to Fan Ning and held her chin, her exposed eyes flashing.
Fan Ning used a dropper to absorb several sections of mixed solvent, squeezed it out from the mouth of the column in a circle around the inner wall, and gradually melted the previously poured silica gel powder into a flocculation like the silica gel at the bottom.
"Remember, you have to squeeze it in in a circle like I did, and don't drop it directly, otherwise it will make holes on the surface of the sample and affect the separation purity."
When the liquid above the sample had reached a certain height as a buffer, Fan Ning connected the glass solvent ball with the upper and lower ends open above the column mouth, fixed it with a clamp, and began to pour a large amount of solvent into it.
Then, he closed the top glass stopper and turned on the mini steam blower connected by a rubber tube.
The solvent starts to flush the silica gel column from top to bottom, and starts to drip from the bottom, and the flask takes over.
During this period, Fan Ning continued to use a capillary glass tube to absorb the liquid at the outlet, and then spotted it on the silica gel surface of the thin layer chromatography, placed it on the altar crystal ball and checked.
"You are watching when the first component flows out of the solvent, right?" Joan added some radiant spirit liquid to the altar, and then asked, "If I understand correctly, the mixture has begun to move downwards.
It just takes some time.”
There was a hint of approval in Fan Ning's eyes.
This Miss Joan Nehemiah is very perceptive. In her previous life, she must have been my favorite junior sister in the research group.
More than a minute later, Fan Ning looked through the crystal ball and saw the iterative black and white light and shadow on the silicone surface.
The extended test tube rack at hand was already filled with 60 test tubes. He removed the Erlenmeyer flask, switched to test tubes and began to pick them up one by one.
"Why not continue to use the Erlenmeyer flask?" Joan asked.
"Because I don't know when the 'pool' will flow out." Fan Ning explained, "If I use an Erlenmeyer flask, by the time I find the light and shadow of the 'pool' in the altar, it has already dripped and will be contaminated again.
'Derivation'... Therefore, we generally default to using test tubes to collect the first component from the outflow. This not only facilitates operation, but also ensures that cross-contamination only occurs in a part of it at most..."
"Now, please help me conduct thin layer chromatography on test tubes 1-5 in order to confirm the components." He put forward a task to Qiong.
Qiong followed the method he taught, using a pencil to draw lines on the thin layer chromatography plate, marking the numbers, and spotting the capillary tube. The lower end was immersed in the developing agent of the glass bottle, climbed up, and then clamped it out, and observed it in the altar.
"Report to Professor Fan Ning, a row of 'evolved' light and shadows of the same height."
"Put them together with the liquid in the Erlenmeyer flask, and evaporate the solvent on the rotary evaporator. Remember to recycle the solvent and put the proportion label on it," Fan Ning said.
"Okay!" Joan's tone was very excited.
A few minutes later, she got a large drop of silver-white viscous liquid that was as mercury-like in the bottle. According to Fan Ning's request, she sealed it and put it in a lead box.
During the thin-layer chromatography of the liquid in the sixth test tube, Fan Ning discovered that there were pink and black and white stripes of light and shadow on the "runway", so he put a disposal label on it, indicating that he would only recycle the solvent and not
Keep its ingredients.
Test tubes No. 7-18 have a single pink "pool" in phase. After the solvent is evaporated, a bunch of strange substances in the shape of flowers are obtained.
At this time, Fan Ning began to switch to a solvent with a volume ratio of cyclohexane:coelomatous lymph fluid of 15:1, and added two drops of beetle instar passivation ester.
Test tubes No. 24-34 have a single "cocoon" phase. After evaporating the solvent, a beautiful oval green crystal was obtained.
Change the solvent ratio to 5:1 and add 5 drops.
Test tubes No. 37-50 have a single "candle" phase. After the solvent is evaporated, a pool of constantly dancing golden liquid is obtained.
At nearly eleven o'clock in the evening, all four extraordinary components were separated by Fan Ning.
The two girls looked at him as if they were monsters.
"Caron, I can't understand how your brain grows anymore..." Sheeran stared at his face intently. "Maybe you can also become an honorary associate professor in the Chemistry Department of the Polytechnic Institute."
"No... with this achievement alone, he might be able to become the honorary dean of the Institute of Science and Technology." Qiong shook her head seriously, "Do you know how difficult it is to separate extraordinary components? All the elixir masters are here
This gives me a headache. I can't estimate its value at all. Just the detection method of 'thin layer chromatography' is enough to make a big change in the working methods of elixir masters. Moreover, 'chromatography' seems to be a system. It simply has no
Value cap…”
"Caron, how did you come up with this? Is it the mystical revelation in the dream? Did the 'key' you studied bring it to you?" Joan, wearing a mask, approached Fan Ning, her dark eyes blinking.
.
"When I was in an art museum before, I accidentally noticed the diffusion of mixed pigment solutions on paper..." Fan Ning briefly explained the origin of this chromatography phenomenon and the principle of chromatography that he "speculated".
Qiong listened and said "Yeah" seriously, then turned around and said: "Xilan, we have to be cautious and cautious. These extraordinary components may not be anything in themselves, but this separation method and effect must not flow out..."
Her voice was low and she looked around nervously at the doors and windows.
"This is the first time I've seen you remind others to be careful." Xilan's smile was filled with playful relief.
Joan didn't take her teasing seriously and continued to say seriously: "...Carolon, I still don't know many details. As long as you teach me later, I can keep washing bottles for you..."
"That's not the case, let's wash them together..." Fan Ning gently put the used glass bottles and test tubes into the sink one by one. "It's already very late, and time is tight. Next, we still need to roughly figure out these extraordinary things."
The mysterious properties of matter.”
"In this way... combined with their origins, we may be able to deduce many clues related to them..."