Chapter 290: Penicillin...Grow the penicillium first!
Gretel was extremely happy. He rushed out of the anatomy room on the basement floor and ran to the P4 laboratory upstairs. He began to cast blessing spells on himself one by one:
Oil the soles of your feet!
The lightness of cats!
Jump!
"That's enough for you!" Behind him, Master Lin En was carrying his robe and ran one floor before he was thrown away half a floor. Looking at Gretel, he couldn't help shouting:
"There are only two floors in total! You have cast three spells!!!"
With a meow, the black cat jumped up, expanded, expanded, and expanded again in mid-air, turning into a giant black panther with a shoulder height of 1.8 meters. He lowered his head, picked up Master Lin En's collar, and walked up the stairs.
He rushed over.
Gretel really couldn't be unhappy. From the time he arranged this research project, from the time when he was still at the original site of the laboratory, searching for melons all over the world, or even earlier, from the time he saved the old dwarf in the forging area, from the time when the old dwarf's sputum
When the Mycobacterium tuberculosis bacilli was cultured, he was waiting for this day——
penicillin!
Or, other antibiotics that can secrete lysozyme! Streptomycin, oxytetracycline, or even some gentamicin would be good!
The only things that could stop him were the caution of a doctor and the fear of a massive leak of infectious bacteria as a researcher!
Even though Grete's hands were shaking with excitement, he followed the safety operating procedures and meticulously wore a mask, gloves, washed hands, and put on protective clothing. After all the students had finished wearing them - by the way, Mr. Troca was carried by the golden skeleton as usual
With his skin on the back of his neck, he rushed to the door - he opened the door, entered, closed the door, opened the inner air-tight door, and entered the core laboratory layer by layer.
The P4 laboratory in the Mage Tower has been greatly modified compared to Gretel's original version. One particularly important point is that the original single channel has been made into three areas and two channels, animal culture area, anatomy area, and bacteria area.
The culture area, experimental area, and each room are independent, with clean channels in and contaminated channels out.
Do you want to enter the red zone and then retreat to the clean corridor like the last time the judges came to visit?
Impossible, the door has one-way access and is controlled by the tower spirit!
The large group of people rushed straight to the bacterial culture area. Under the guidance of the reporter student, Gretel picked up the petri dish and looked at the light carefully. There was no need to take samples, prepare slides, stain, or use a microscope. He relied on his naked eyes to
I saw something strange on the petri dish:
Among the pale yellow bacterial colonies that covered the bottom of the petri dish, there was a large area that was translucent in appearance, like glass. Outside the blank area, large areas of Staphylococcus aureus were crowded together, but they failed to make a step forward;
In the center of the blank area, a small piece of green mold stands proudly and proudly!
that's it!
This is what I want!
Gretel suddenly turned around. Even though he was wrapped in a protective suit and his voice was buzzing, he couldn't stop him from raising his voice excitedly:
"Which group conducted this experiment?"
"Our group!"
A clear female voice answered. Gretel turned his head and saw a tall student, only half a head shorter than himself, stepping out of the crowd and standing in front of him. Considering that there were only two women in this batch of apprentices, and the other one was 1 meter tall.
The little man named Wu, no need to ask, must be the one who brought the wild boar here.
"Very good." Gretel nodded heavily, trying hard to suppress his intense heartbeat:
"Your group will pause your previous work and concentrate on studying these green things in the petri dish.
——I want you to cultivate it, purify it, find out why it makes bacteria afraid to get close, and prepare a large amount of that substance!
Everyone, congratulations, you may have found a new way to restrain the plague!"
In the room, all 24 students were shocked. Especially the eight people from Black Crow Swamp, their breathing became heavy and they took a step forward reflexively.
It's a pity that even if their eyes are green, not everyone can participate in the project...
The third batch of students who passed the assessment were defined by Grete as poor students and were not qualified to participate in the project at all. They could only work hard and practice more, hoping that if there is a new exam next time, they could turn around in one fell swoop. Just this one step would make them successful.
1/3 of the people were eliminated.
Not everyone can touch the first and second batches. Among the ten people who passed the examination in the first batch, the five who were infected with Mycobacterium tuberculosis were left to do whatever they were supposed to do——
Mycobacterium tuberculosis is not sensitive to penicillin but is sensitive to streptomycin. These five people, as well as the second batch of three people who passed the examination and joined their work, have to continue to work hard. Want to do something new for a change?
Thinking too much!
In the end, only the young lady from the Natural God Church, with two necromancers, a medical magician, a God of War priest, and three people who joined later, did a great job of making penicillin. According to the young lady's instructions, every day
Work in a group, first make various culture media, and cultivate in large quantities——
Then, Gretel spent two days in a row listening to their wailing in various styles.
"I'm dying..."
"Kurona, I'm done with this too... give me some more..."
"No, it's not possible. It's half-dead... Corona, why are you raising it so well..."
"Can't we necromancers keep this kind of thing? There is too much death aura on it! It can't survive with anything! Let me tell you, when I was at home, I couldn't even keep catnip alive!"
"Don't talk nonsense, didn't you feed the bacteria?"
"Bacteria are plagues! Isn't it right for necromancers to feed plagues? But this thing is not a plague! It is to restrain the plague! It's normal if I can't feed it. If I can feed it, I will be killed by it.
Got it!"
Gretel snickered beside him. How can it be so easy to cultivate Penicillium? The nutrient ratio of the culture medium is wrong, the pH is wrong, and the oxygen content of the air is wrong. It will either not grow, grow less, or not ferment.
Less fermentation. If there is no fermentation, it will produce less penicillin, and it will be a waste of time...
In the history of the previous life, Alexander Fleming discovered penicillin in 1928, and after five years of hard work, he turned to sulfa;
In 1938, German chemist Ernst Chain began purification experiments, and by the winter of 1940, a little bit of penicillin was refined;
In 1941, Australian pathologist Florey increased the production of penicillin from 2 units per cubic centimeter to 40 units;
It wasn't until 1942 that mass production of penicillin began. So in those spy movies, the rush for penicillin began during the Long March. Well, I suggest the crew turn their target to sulfa...
So many of the world's top scientists worked hard to create penicillin that could be mass-produced. If Gretel hadn't had the support of magical powers to work out miracles, he wouldn't have dared to take part in this project.
All in all, come on, boys and girls!
****
I’m so tired, I just wrote 4,000 words today…
There is one extra update left, I will pay it back... I will definitely pay it back...
And worship, Fleming, the man who discovered penicillin, was such a strong man! He worked at the age of 16, and received an inheritance of 250 pounds at the age of 20. His brother suggested that he study medicine. Then, in July of that year! Note that it was that year! He passed 16 courses.
Exam! Enter the University of London!
You asked me to take a year off from work to review after four years of work, and I couldn’t even pass six courses...