Chapter 291 Penicillin Experiment—Penicillium was stolen!
The miraculous plant growth technique - no, it was microbial growth hormone, gave Grete the confidence to launch the penicillin project. And this magical technique did not live up to his expectations. After a week, enough Penicillium was reproduced.
The point of doing experiments.
As for the priest apprentices of the Natural God Church, the rats they bred were enough for them to conduct a luxurious experiment...
In the anatomy room on the first floor of the Master Tower, 24 apprentices were divided into six experimental tables, each facing a cage of rats. In the crowd, two teenagers from the Natural God Sect looked nervous and whispered:
"Use it sparingly...Use it sparingly...Don't kill yourself by being careless..."
It's so pitiful that their experience of raising rats makes everyone cry when they hear it and saddened when they see it. There are no specialized breeding companies. There is no specially prepared mouse feed. The condition is stable and there are no germs. They have survived for several days in the laboratory.
There is no such thing as a modern mouse...
We have to rely on the priests of the God of Nature to dig out mouse holes, catch them, and let the mother mice give birth to pups, and raise them after they give birth. The mice are frightened and frightened, so they use animals to comfort them; they don’t know the feed ratio, and their animal communication skills are top notch.
Above; if you want to be free from germs, practice healing skills every day. The priest's apprentice's healing skills are limited, and he is reluctant to use them even though he is struggling, and all of them fall on the rats.
It is not an exaggeration to say that the mice raised in this way are the lifeblood of the priests. Now they are used for experiments, and 120 of them are used at one time. It is simply!
However, raising rats lasts for a hundred days, and using rats only lasts for a while. Gretel ordered them to be used, but they could not resist. The four priest apprentices could only gather around the table and watch Gretel demonstrate to the students with sad eyes:
Lift the rat's tail with your right hand and place it on the lid of the rat cage;
When the mouse struggles to crawl forward, use your left thumb and index finger to grasp the mouse's ears and neck skin;
With the mouse in the palm of your left hand, use the ring finger and little finger of your left hand to pinch its back skin and tail...
"Do you understand everything? The movements must be steady and accurate. If you don't catch it well once, you have to let go and catch it again. Be careful of being bitten by it! - Come on, everyone can catch one by themselves!"
This sentence was like pouring a ladle of cold water into a boiling oil pan. In an instant, the entire anatomy room was noisy. Of the 24 students, 20 were shouting in surprise:
"Ouch! It bit me!"
"Run away! Run away!"
"Mr. Trocca! Help!—No, don't kill it!"
"Ahhh! I squeezed the shit out of it!"
The four priest apprentices of the God of Nature looked at each other. Catching mice can also be a problem? Isn't it just a matter of reaching out and grabbing it?
Gretel was already prepared for this. Well, when he first started doing animal experiments, his classmates in the same class also looked like this, no better than these spell casters. With 120,000 yuan of patience, he continued
Set up a table and look at it, giving instructions one by one:
"Be quick when grabbing the tail! Be accurate!"
"No, don't scratch the base of the tail! Grab the middle part of the tail!"
"It's not like you have to use so much force... Do you think you're going to have to break off your tail?"
"If it doesn't work, just carry it around a few times to make it faint... Hey, why did you throw it away on your own! Mr. Trocca, please help to catch it!"
"Then pinch its ears...don't pinch the neck! Don't press! Don't pull!!!"
With a click, the mouse died...
Grete stroked his forehead, pulled the mouse's tail, and pressed its cervical vertebrae at the same time. This is the way to kill a mouse, classmate! I haven't taught you this lesson yet!
"Okay, you can catch another one...Fortunately, I have a spare supply..."
Just the act of catching the mouse went through all kinds of people's ups and downs (in which Mr. Trocca was very popular). Finally, after stumbling, the experiment progressed to subcutaneous injection of the mice and inoculation with Staphylococcus aureus.
stage.
Grete once again demonstrated for them. It was obviously a very simple subcutaneous injection action - much simpler than intraperitoneal injection, intracranial injection, etc. - and had to patrol the tables again to correct all the wrong behaviors of the apprentices:
"Keep your hands steady! The more the mouse struggles, the more steady your hands must be! - Stop!... It's too late, you'll hurt yourself..."
"Don't squeeze it so hard! The mouse can't escape! ...Forget it, it's dead after squeezing it, just change it!"
"Insert the needle flat! Don't be vertical! Don't be crooked! - Look, you pricked your finger!"
"Don't insert the needle too deep! Wait--" The blood is so fast! Did you puncture a major artery or something?
"Subcutaneous! Subcutaneous! Insert the needle flatly, and then lift the needle upward! Lift it flatly, not the needle pointing upward! Oops, the skin has been poked through... Forget it, put the needle in another site!"
This kind of thing can be made by skilled masters with ease and ease, but by novices who don't know how to make it in a variety of ways. Even though Grete was fully prepared mentally, he was still confused by them, and his blood pressure soared to 20 mmHg.
It was difficult to complete the hypodermic injection experiment, during which 10 mice were killed, four students poked their fingers, overturned penicillin test tubes, broke syringes and other countless accidents. In the end, 80 mice entered alive.
Entering the observation stage...
"How's the mouse doing today?"
"normal."
"normal."
"Mine is normal too."
The students were calm. The next day the mouse was in normal condition, and the third day...
"It doesn't eat much anymore!"
"I don't like to move anymore!"
"Looking so lethargic!"
On the fourth and fifth days, the mice's condition worsened. By the sixth day, at least half of the groups reported that the mice's condition had worsened, and their faces gradually turned blue and continued to deepen.
"It's belly is also blue! Where the needle went in, it's all blue!"
"Sir, is it dying?"
Four priest apprentices from the Order of the God of Nature and two apprentices from the Temple of the God of War looked at Gretel eagerly, just waiting for his words. Gretel took a deep breath:
"Not necessarily.—Bring us the penicillium we prepared!"
They divided the sick mice into four groups. One group was given oral administration of Penicillium, one group was injected with Penicillium subcutaneously, and one group was treated with magic... One group did nothing and was left alone as a control. The apprentices wished they could grow up in the laboratory.
, eyes wide open, staring at the mice...
"Oral Penicillium group. This one won't work..."
"Oral group. This one seems to be no longer good." A mage picked up the mouse and observed it. The priest of the God of Nature turned his face sideways and glared at him. The mage shrugged:
"What's wrong? I am a necromancer. Although we are not compatible with animals, I can feel the breath of death very strongly!"
"Hypodermic injection group.—Hey, sir, this one seems to be doing better!"
There was a crash, and 25 of the 24 students in the entire anatomy room suddenly gathered around. The one with the largest number was Master Lin En. He stretched out his two skinny arms on the outside of the crowd and squeezed in:
"Yes, yes, it is indeed better! The aura of death is not so strong!"
One day later, 18 of the 20 mice in the subcutaneous injection group were alive and kicking and escaped death. This therapeutic effect was second only to the magic group and far higher than the oral group and the control group, all of which died.
"Congratulations, ladies and gentlemen." Gretel clapped his hands and attracted the attention of the students:
"You have successfully found a natural product that can effectively treat patients in places where magic cannot reach. Now, let us continue to study, find out the appropriate dosage of this product, and make it into a potion
, how to carry it long distance!”
"yeah!"
"Long live!"
"We made it!"
More than twenty surgical caps flew up to the ceiling. White, gray, blue——
As for the material of the surgical cap, Grete allowed them to use it freely, as long as it could tightly wrap the hair and not hinder the operation. So, during this wave of celebrations, he also saw two touches of green, one falling together and one falling together.
fall……
After a short celebration, the students entered the long experimental process again. This time, more culture media were produced to promote the growth of Penicillium - sugar agar culture medium, blood agar culture medium, beef extract culture medium, and wort culture
Base, corn meal medium, potato sucrose medium...
The entire laboratory, no, the entire mage tower, seems to have become a big kitchen. Steaming, boiling, boiling, crushing, filtering...it is steaming all day long. If the students don't want to eat, they can just drink the culture medium.
Fill the stomach.
Of course, no one actually does that. In the words of Leon Carlos:
"I know that wort smells bad, but I didn't know that beef extract smells worse than it...it's disgusting, this smell..."
Gretel allowed the students to use their imagination as much as possible and toss in various formulas. Waste is certain, but fortunately there is a fire element pool in the mage tower, which provides endless heat; those sea stone flowers, beef, and sheep blood
, you can buy a lot of money with just one contribution point, so you don’t have to worry about not being able to afford it. Corn, potatoes, etc.?
Just kidding, that thing is not worth the money!
During the mass production boom, the four priest apprentices of the God of Nature have become the leaders of the students. Especially the girl who caught the wild boar, she has basically stopped doing culture media and spends two hours every day walking around the experiment
In the room, with half-closed eyes, I sensed the petri dishes one by one:
"This... doesn't work. It's basically not long."
"This...it grows a little slowly, you can keep an eye on it."
"This one grows up pretty fast."
"This...can you raise the temperature a little?"
I have to say that you can always trust the biological affinity of the Priest of the God of Nature. Of course, except for the fake Gretel. After ten days, they have screened out all meat and blood raw materials and began to specialize in corn flour.
After reaching this point, more than half of the 24 students were so tired that their eyes were crooked, their mouths were crooked, and their facial features were abnormal. Grete himself felt a little overwhelmed, so he waved his hand generously and allowed them to go home and relax:
"Have a good rest for two days. When we come back, we will start a new project!"
The students cheered and dispersed. Gretel couldn't wait to rush out of the mage tower and go to the beach to breathe fresh air. Just kidding, he has been working on experiments for so many days and he hasn't even been exposed to the sun. If he doesn't go out for a walk, he will be deficient in vitamins.
D!
Gretel played happily for most of the day. When he returned to the mage tower, he received a blow on the head:
Penicillin strains were stolen.
****
Today I asked in the group what accidents have occurred when you did subcutaneous injection experiments on mice, and then I heard all kinds of blood and tears...
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